01/

Plasma cell-free RNA PD-L1 Expression and Clinical Outcomes with Immunotherapy in Advanced NSCLC

Paul R. Walker, Sriraksha Jayananda, Mahvish Muzaffar, Praveen Namireddy

04/

A multi-biomarker approach to defining the oligometastatic state using liquid biopsies

Vasudeva Bhat1, Rohann J. M. Correa2, Aisling Barry3, Houda Bahig4, Darin Gopaul5, Andrew Pearce6, Famke L. Schneiders7, David A. Palma2 and Alison L. Allan1,2

07/

Hepatocellular carcinoma risk-stratification based on ASGR1 in circulating epithelial cells for cancer interception

Amparo Roa Colomo a, b, María Ángeles López Garrido c, Pilar Molina-Vallejo d, Angela Rojas e,f, Mercedes González Sanchez c, Violeta Aranda-Garcíag, Javier Salmeron b, , Manuel Romero-Gomez e,f, Jordi Muntane f,h,j, Javier Padillo j, Jose María Alamoj, Jose A. Lorente d,k, María José Serrano d,l, m  and M. Carmen Garrido-Navas d,n

10/

Transcriptional Patterns of Genes Encoding Blood-Secreted Molecules Predictive of Survival in Hepatocellular Carcinoma and Cholangiocarcinoma

Paula Doria, Jaime Navarro, Belén Bolinches, Sandra López-Domènech, Alejandro Herreros-Pomares, Fernando Vidal-Vanaclocha

13/

Study of soluble markers as predictive and prognostic biomarkers to immunotherapy with pembrolizumab in first line treatment of advanced Non-Small Cell Lung Cancer (NSCLC)

S. Torres Martínez1, A. Moreno Manuel1, S. Calabuig-Fariñas3, M. Ferrero Gimeno1, S. Gallach1, M. Mosqueda1, A. Blasco Cordellat4, Clara García4, F. Aparisi4, E. Jantus Lewintre5, C. Camps6.

16/

Stabilization of Urinary cfDNA with Novel UAS Chemistry to Facilitate Post-Collection Sample Transportation and Storage

Cameron Wood1, Kyle MacDonald1, Thanh Dang1, Tara Crawford Parks1, Graham Morse1, Rafal Iwasiow1

19/

Platelets Lead Morphological, Phenotypic and Functional Changes in Tumor Cells

Alba Rodriguez-Martinez1,2†, Iris Simon-Saez1,3†, Sonia Perales1,3, Carmen Garrido-Navas1, Alessandro Russo4, Diego de Miguel-Perez4, Ignacio Puche-Sanz1,5, Clara Alaminos6, Jorge Ceron1,3, Jose A. Lorente1,2, Massimo Cristofanilli7, Alba Ortigosa-Palomo1, Pedro J. Real1,3,8, Christian Rolfo4*, María J. Serrano*1,9,10

22/

Extracellular vesicle-contained TGF-β as a predictive biomarker in non-small cell lung cancer patients undergoing immune-checkpoint inhibitors.

Diego de Miguel Perez1,2, Alessandro Russo2,3, Muthukumar Gunasekaran2, Feliciano Barrón4, Luis Lara-Mejía4, Andrés F. Cardona5, Christine Peterson6, Rivka Colen7, Aung Naing8, Phil Mack1, Fred Hirsch1, Oscar Arrieta4, Vincenzo Adamo3 & Christian Rolfo1,2*.

25/

Development and Analytical Validation of a Highly Sensitive and Specific NAPA Assay for the Detection of ESR1 Mutations in Circulating Tumor Cells and Plasma Circulating Tumor DNA

Dimitra Stergiopoulou1, Athina Markou1, Eleni Tzanikou1, Ioannis Ladas2, G. Mike Makrigiorgos2, Vassilis Georgoulias3,4, Evi Lianidou1

28/

Evaluation of Monocarboxylate Transporters (MCT4 and MCT1) Expression and their Prognostic Significance in Circulating Tumor Cells from Patients With Early Stage Non-Small-Cell Lung Cancer

A. Zafeiriadou1, T. Londra1, V.Tserpeli1, V. Georgoulias2, A. Kotsakis3, E. Lianidou1 and A. Markou1

31/

Multiparametric characterization of CTCs by a novel methodology combining microfluidic isolation (IsoFlux) and imaging flow-cytometry (ImageStream)

Inés Aznar-Peralta 1,5* , Antonio J. Ruiz-Rodríguez 1,2 , M. Pilar Molina-Vallejo 1 , Cristina
González Puga 3 , Inés Cañas García 3 , Encarna González 4 , Jose A. Lorente 1,5 , M.Jose
Serrano 1,6,7 , M. Carmen Garrido-Navas 1,8

34/

Phase II randomized study of intermittent versus continuous schedule of vemurafenib plus cobimetinib in BRAF-mutated advanced melanoma

Clara Mayo de las Casas1; Maria Gonzalez-Cao1; Juana Oramas2; Miguel A Berciano-Guerrero3; Luis de la Cruz4; Pablo Cerezuela5; Ana Arance6; Eva Muñoz-Couselo7; Enrique Espinosa8; Teresa Puertolas9; Roberto Diaz-Beveridge10; Sebastian Ochendustko11; Maria Jose Villanueva12; Laura Basterretxea13; Lorena Bellido14; Delvys Rodriguez15; Ana Drozdowskyj1; Miguel A Molina1; Jose Antonio Lopez-Martin16; Alfonso Berrocal17; 

37/

BRCA status in circulating free DNA by using the commercial Devyser BRCA kit: a preliminary evidence

Alessia Perrucci1, Elisa De Paolis1, Maria Elisabetta Onori1, Anna Fagotti2, Giovanni Scambia2, Andrea Urbani1,3, Angelo Minucci1

40/

Liquid biopsy monitoring allows detection of disease progression in EGFR-mutated NSCLC patients treated in first-line with Osimertinib

A. Moreno-Manuel1,2,3*, S. Calabuig-Fariñas1,2,3,4*, M. Mosqueda1,2, S. Gallach Garcia1,2,3, E. Escorihuela1,2, M. Bellón1,2, M. Núñez5, M. Ferrero Gimeno1,2,3, S. Torres1,2,3, J. Korsten1, A. Navarro6, L. Navarro6, A. Fernández5, A. Blasco Cordellat3,5, F. Aparisi5, E. Jantus Lewintre1,2,3,7&, C. Camps1,2,5,8&.

02/

Circulating tumor cells (CTC) are the most relevant diagnostic biomarker in transplant-eligible multiple myeloma (MM)

Juan-Jose Garcés, Maria-Teresa Cedena, Noemi Puig, Leire Burgos, Jose J Perez, Lourdes Cordon, Juan Flores-Montero, Luzalba Sanoja-Flores, Albert Ortiol, María-Jesús Blanchard, Rafael Rios, Jesus Martin, Rafael Martínez-Martínez, Joan Bargay, Anna Sureda, Javier de la Rubia, Miguel-Teodoro Hernandez, Paula Rodriguez-Otero, Alberto Orfao, Maria-Victoria Mateos, Joaquin Martinez-Lopez, Laura Rosiñol, Joan Blade, Jesus F. San-Miguel, Bruno Paiva

05/

The role of circulating tumor DNA to predict mutant allelic fraction evolution in non-small cell lung cancer patients: a mathematical model.

Pasquale Pisapia1*, Mauro Buono2*, Francesco Pepe1, Gianluca Russo1, Rossella Tufano3, Christian Rolfo4, Giancarlo Troncone1, Umberto Malapelle1.

08/

Sandwich-Type Electrochemical Paper-Based Immunosensor for Claudin 7 and CD81 Dual Determination on Extracellular Vesicles from Breast Cancer Patients

Francisco Gabriel Ortega Sánchez1, Coral González Martinez1, María José Serrano Fernández1, José Antonio Lorente Acosta1,2, Matías Regiart3, Martín Fernández Baldo3.

11/

The relevance of liquid biopsy for KRAS/NRAS/BRAF baseline testing, and monitoring at disease progression, in metastatic colorectal cancer (NEORAS)

Joseph Zouein, Hampig Raphaël Kourie

14/

Comparison of three commercially available kits for cfDNA isolation from first-void urine collected with Colli-Pee® containing UAS

Jordaens S. 1,2 Zwaenepoel K. 1,3, Vorsters A. 4, Beyers K.2, Deben C. 1, Tjalma W. 5, De Wachter S. 6, Van Dam P.5, Vankerckhoven V.2, Pauwels P1,3

17/

First-void urine self-sampling for hrHPV testing in cervical cancer screening: sample preference from a Belgian triage population

Jhana O. Hendrickx1, Stephanie Jordaens1, Nette Meers1, Koen Beyers1, Vanessa Vankerckhoven1

20/

Early detection of advanced non-small cell lung cancer (aNSCLC) progression by simple, low-cost pipeline for cell free multiplex based liquid biopsy (LB).

Ari Raphael, Victoria Hannes, Dov Hershkovitz, Shlomo Tzuriel.

23/

Cell-free RNA analysis for early detection of complex diseases

Perez Boza, Cristina; Cabus, Lluc; Tuñi, Cristina; Curado, Joao; Lizano, Esther

26/

A ten-year follow-up of a breast cancer patient based on a comprehensive liquid biopsy approach

Dimitra Stergiopoulou1, Athina Markou1, Areti Strati1, Martha Zavridou1, Eleni Tzanikou1, Sophia Mastoraki1 , Stavroula Smilkou1 , Galatea Kallergi2 , Vasillis Georgoulias3 , Prof. Evi Lianidou1

29/

Prognostic significance of DNA methylation in primary tumors and plasma cell-free DNA in high-grade serous ovarian cancer

Victoria Tserpeli1, Dora Londra1, Lydia Giannopoulou1, Paul Buderath2, Ioanna Balgkouranidou3, Nikolaos Xenidis3, Stylianos Kakolyris3, Kitty Pavlakis4, Theodoros Rampias5, Sabine Kasimir-Bauer2 and Evi S. Lianidou1 

32/

USP44 PROMOTER METHYLATION IN PLASMA CELL-FREE DNA IN PROSTATE CANCER

Theodora Londra 1, Sophia Mastoraki1, Evaggelos Bournakis2, Martha Zavridou1, Anastasios Thanos3, Theodoros Rampias4, Evi Lianidou1.

35/

Development and analytical validation of a Reverse Transcription Droplet Digital PCR (RT-ddPCR) assay for PD-L1 transcripts in Circulating Tumor Cells

Areti Strati1*, Martha Zavridou1, Panagiota Economopoulou2, Stavros Gkolfinopoulos2, Amanda Psyrri2, Evi Lianidou1*

38/

Analysis of soluble markers as predictive biomarkers to neoadjuvant chemotherapy in early triple-negative breast cancer (TNBC)

S. Torres-Martínez1, M. Nuñez Abad2, S. Calabuig-Fariñas3, E. Escorihuela1, Macarena Ferrero1, Clara García2, M.J. Godes Sanz de Bremond2, J.Á. García García5, V. Gumbau Puchol6, E. Jantus Lewintre7, V. Iranzo2, C. Camps8.

41/

Precision medicine in cancer: platforms for targeted therapy selection based on liquid biopsy

Lorena Alonso-Alconada1, Eva Cagide2, Óscar Cascón2, Lorena Diéguez3, Paulina Piairo3, Sergio Figueiras4, Cristóbal Bernardo Castiñeira4, Carmen Álvarez4, Manuel Suárez4, Rafael López-López5, Eladio Crego1 and Miguel Abal5.

03/

Detection of circulating tumor DNA for molecular characterization and disease monitoring in metastatic colorectal cancer patients

Milena Urbini1*, Giorgia Marisi1*, Alessandro Passardi2, Chiara Molinari1, Matteo Canale1, Laura Matteucci2, Giulia Bartolini2, Gianluca Tedaldi1, Irene Azzali3, Davide Angeli3, Giovanni Luca Frassineti2, Paola Ulivi1.  *equally contributed

06/

Feasibility of BRAF mutations testing in Non-Small Cell Lung Cancer on Liquid biopsy samples: focus on basal setting.

Francesco Pepe, Pasquale Pisapia, Antonino Iaccarino, Roberta Sgariglia, Mariantonia Nacchio, Gianluca Russo, Gianluca Gragnano, Caterina De Luca, Giancarlo Troncone, Umberto Malapelle.

09/

New approach to screening colorectal cancer patients by Liquid Biopsy; Differential presence of Exons (PDE)

D. Rubio-Mangas, Y. Torres Rodríguez, M. Carcajona Mata, J. Suela Rubio, M. García-Arranz, D. García-Olmo.

12/

TARGETED NGS USING LIQUID BIOPSY TO PERFORM MOLECULAR DIAGNOSIS AND PATIENT MONITORING IN NSCLC

A. Moreno-Manuel1,2,3*, S. Gallach Garcia1,2,3*, S. Calabuig-Fariñas1,2,3,4, A. Blasco Cordellat3,5, M. Ferrero Gimeno1,2,3, V. Ruiz Cordero5,  S. Torres1,2,3, M. Mosqueda1, L. Navarro6, F. Aparisi5,  C. González5, E. Jantus Lewintre1,2,7&, C. Camps1,2,4,8&

15/

CfDNA vs Extracellular Vesicle-Derived DNA for the Detection of Colon Cancer

Kavita Thakur 1 2, Manu Smriti Singh 3, Sara Feldstein-Davydova 1 2, Victoria Hannes 1, Shlomo Tsuriel 1, 4 and Dov Hershkovitz 1 2, 5,

18/

Enumeration of Circulating Endothelial Cells in Systemic Sclerosis through CellSearch System: a new approach to CECs determination

Francesca Pignataro*1, Laura Zorzino2, Antonina Minniti1, Wanda Maglione1, Marco Picozzi2, Giulia Clericuzio2, Cecilia Simonelli3, Francesco Picardo1, Roberto Caporali1,4, Nicoletta Del Papa1

21/

Plasma microRNAs for early detection of cerebellar atrophy caused by PMM2-CDG

Lluc Cabus1, Cristina Tuñi1, Cristina Hernando-Davalillo2,  Adrián Alcalá2, Mercè Bolasell2, Patricia Fernández-Pareja2, Gisela Márquez2, Gregorio A. Nolasco3, Joao Curado1, Jennifer Perez-Boza1, Mercedes Serrano3, Esther Lizano1

24/

ALK fusions can be clearly detected in extracellular vesicles from NSCLC cell lines. A become of hope for non-invasive ALK testing

Estela Sánchez-Herrero*1,2, Carmen Campos-Silva*3, Yaiza Cáceres-Martell3, Sandra Sanz-Moreno1, Roberto Serna-Blasco1, Alejandro Rodríguez-Festa1, Dunixe Ares Trotta1, Paloma Martín-Acosta4, Cristina Patiño5, María José Coronado6, Alexandra Beneitez7, Ricardo Jara7, Alberto Cruz-Bermúdez1, Víctor González-Rumayor2, Mar Valés-Gómez3¥, Mariano Provencio1,8 and Atocha Romero1,8¥.

27/

Detection of EGFR Mutations in Plasma cfDNA and Paired CTCs of NSCLC Patients before and after Osimertinib Therapy Using Crystal Digital PCR

Aliki Ntzifa1, Athanasios Kotsakis2, Vassilis Georgoulias3, Evi Lianidou1

30/

The Effect οf Genomic DΝΑ Contamination on the Detection of Circulating long non-coding RNAs: The Paradigm of MALAT1

Stavroula Smilkou 1, Emilia Tsaroucha 2, Evi Lianidou1, Athina N. Markou 1*

33/

Combination of LAG3 and HVEM as a predictive biomarker to second-line immunotherapy treatment in advanced Non-Small Cell Lung Cancer (NSCLC) patients

S. Gallach Garcia1*, S. Torres Martínez1*, J. Garde2, S. Calabuig-Fariñas3, M. Ferrero Gimeno1, A. Moreno1, A. Blasco Cordellat4, F. Aparisi4, E. Escorihuela5,  C. Camps6&, E. Jantus Lewintre7&.

36/

A comprehensive molecular analysis of in-vivo isolated EpCAM-positive circulating tumor cells in Breast Cancer

Areti Strati1, Martha Zavridou1, Galatea Kallergi2,3, Eleni Politaki2, Andra Kuske4, Tobias M. Gorges4, Sabine Riethdorf4, Simon A. Joosse4, Claudia Koch4, Anna-Lena Bohnen5, Volkmar Mueller5, George Koutsodontis6, Emmanouil Kontopodis7, Nikiforita Poulakaki8, Amanda Psyrri6, Dimitris Mavroudis2,7, Vasilis Georgoulias2, Klaus Pantel4, Evi S. Lianidou1

39/

Developing and implementing NGS-based liquid biopsy molecular profiling in
patients with breast, colorectal and lung cancer in Andalusia’s Health Public System
(SAS).

Inés Calabria, Yania Yáñez, Pablo
Gargallo, Inmaculada González, MD Sequedo, Jennifer Valero, Carlos Ruiz.